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Article Snippet: DAPI , Abcam , ab104139.

Techniques: Recombinant, TUNEL Assay, Apoptosis Assay, Lysis, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software, Confocal Laser Scanning Microscopy

Journal: Human Genetics

Article Title: De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome

doi: 10.1007/s00439-020-02175-x

Figure Lengend Snippet: Immunofluorescence analysis performed on HEK293 cells ( a ) and human fibroblasts ( b ). The N-terminal antibody detected isoforms 1, 3.1 and 3.2 in the cytoplasm and nucleus. Interestingly, an association with centrosomes (white arrow) and kinetochores was detected. Staining with a C-terminal antibody, detecting the full-length isoform 1 and additional hypothetic short C-terminal isoforms, showed a mainly nuclear pattern without a co-localization with the mitotic spindle, centrosomes or kinetochores. α-Tubulin was used for cytoskeletal staining and nuclei were stained using DAPI. Images were obtained using a confocal laser microscope with × 600 magnification, and an additional software magnification as indicated in the respectively images

Article Snippet: Subsequently, cells were washed twice and mounted using FluoroshieldTM with DAPI (Merck Millipore).

Techniques: Immunofluorescence, Staining, Microscopy, Software

Journal: Nature Communications

Article Title: Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer

doi: 10.1038/s41467-022-31769-4

Figure Lengend Snippet: UMAP projections showing the expression of a HLA-I and HLA-II in the CD45 − fraction. HLA-I and II expression was measured by summing the expression of all the individual genes assessed in the locus. b Comparison of HLA-II and CD74 (invariant chain) expression ( R = 0.751, unadjusted p = 0, Pearson correlation test). c Boxplots show the log-transformed expression of the individual HLA-II genes assessed ( p = 1.01 × 10 −178 , Kruskal–Wallis, n = 1377 cells). The box represents the interquartile range, with the center line at the median. The whiskers extend up to 1.5 times the interquartile range (IQR). d Representative multiplex immunofluorescent staining of CD8 (blue), CD4 (red), GZMB (green), PanCK (magenta), HLA-DR (cyan), and DAPI (gray) in an NSCLC tumor ( n = 2). Overlay without DAPI is shown for CD8, CD4, and GZMB, and for PanCK and HLA-DR, along with the corresponding H&E. Scale bar, 25 μm. Source data are provided as a Source Data file.

Article Snippet: Slides were then mounted using Fluoroshield with DAPI (Immunobiosciences) and coverslips applied prior to scanning at 20× using the Leica Versa scanner.

Techniques: Expressing, Comparison, Transformation Assay, Multiplex Assay, Staining